File:Summary of RNA-Seq.svg

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Summary

ᏄᏍᏛ ᏗᎧᏃᏗ
English: Within the organisms, genes are transcribed and spliced (in eukaryotes) to produce mature mRNA transcripts (red). The mRNA is extracted from the organism, fragmented and copied into stable ds-cDNA (blue). The ds-cDNA is sequenced using high-throughput, short-read sequencing methods. These sequences can then be aligned to a reference genome sequence to reconstruct which genome regions were being transcribed. This data can be used to annotate where expressed genes are, their relative expression levels, and any alternative splice variants.[1]
ᎢᎦ
ᏅᏓᏳᏓᎴᏅᎯ Own work
ᏧᏬᏪᎳᏅᎯ Thomas Shafee
Other versions

Also in series:

  • RNA Microarray
    RNA Microarray
  • RNA-Seq
    RNA-Seq
  • SAGE
    SAGE

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  1. ↑ Lowe, Rohan (2017-05-18). "Transcriptomics technologies". PLOS Computational Biology 13 (5): e1005457. DOI:10.1371/journal.pcbi.1005457. PMID 28545146. PMC: PMC5436640. ISSN 1553-7358.

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depicts ᎩᎵᏏ

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inception ᎩᎵᏏ

5 ᏚᏂᏅᏗ 2016

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ᎢᎦ/ᎠᏟᎢᎵᏒᎤᏍᏗ ᏓᏟᎶᏍᏛᎢDimensionsᎬᏗᏍᎩᏍᏆᎳ ᎪᏪᎵ
current12:20, 13 ᎧᎦᎵ 2017Thumbnail for version as of 12:20, 13 ᎧᎦᎵ 20171,020 × 803 (246 KB)Evolution and evolvabilitytext as paths to avoid artefacts
12:19, 13 ᎧᎦᎵ 2017Thumbnail for version as of 12:19, 13 ᎧᎦᎵ 20171,020 × 803 (90 KB)Evolution and evolvabilitytext as paths to avoid artefacts
10:48, 13 ᎧᎦᎵ 2017Thumbnail for version as of 10:48, 13 ᎧᎦᎵ 20171,020 × 803 (88 KB)Evolution and evolvabilityupdate sequence text to 'sans-serif' open font for more robust rendering
08:41, 10 ᎧᎦᎵ 2017Thumbnail for version as of 08:41, 10 ᎧᎦᎵ 20171,020 × 803 (87 KB)Evolution and evolvabilityreverse order of fragmentation and reverse-transcription
05:08, 5 ᏚᏂᏅᏗ 2016Thumbnail for version as of 05:08, 5 ᏚᏂᏅᏗ 20161,020 × 803 (85 KB)Evolution and evolvabilityUser created page with UploadWizard

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